Product presentation
FDISCO method combined with illumination imaging can realize nerve and vascular imaging of mouse brain, muscle, kidney and other complete organs. Because of its excellent clearing and fluorescence retention ability, FDSICO method has obvious advantages in transparent imaging of large biological tissue. Moreover, it has great application value in long-term repeat imaging and weak fluorescence signal imaging of transparent biological tissue.
The specification:
FDISCO in vitro clearing reagent developed by Jarvis has the advantages of fast transparent speed, good transparent effect and strong fluorescence retention.
Directions for Use
Instructions for use of the FDISCO in vitro clearing reagent
1. Composition appearance: This product is divided into 6 reagents, reagents A-F are colorless transparent liquid
2. Main composition: Mainly THF, DBE, water and so on
3. Storage: Keep away from light at room temperature, shelf life is 6 months, please use as soon as possible after opening the cover
4. Operation instructions:
4.1 Sample soaking time:Soak the samples in alphabetical order, and the soaking time in reagents A-E depends on sample size.Reference standard: the whole mouse brain, each reagent immersion time is 12 hours;mouse spinal cord, each reagent soaked for 1 hour.
For large samples, such as the whole mouse brain, an additional reagent E treatment step is required;The immersion time of the sample in reagent F shall be determined by the sample transparency,soak until transparent
4.2 Each of the above steps must be oscillated at constant temperature at 4-8°C and out of light
4.3 The sample should be placed in 5-15ml brown vial, and the amount of reagent should be twice as much as that of the original sample at each step. If the original sample needs 5ml, it is better to add 10ml transparent reagent (the average single dose of each sample is 4-10ml, and the specific amount depends on the sample size)
4.4 After fixation with 4% PFA, the samples should be thoroughly cleaned with PBS (>12h) and then transparent
4.5 Sample should be immersed in MACS-R2 reagent when it is imaged.
