Product presentation
Jarvis' independent r&d in vivo clearing reagent can make skin, bone and other tissues transparent, can be combined with near-infrared imaging, laser speckle imaging, two-photon imaging, Raman imaging, fluorescence imaging and other optical detection technology,and then can obtain non-invasive high-resolution images of nerves, blood vessels and cells under the skin and skull, greatly improve the resolution and imaging depth of all kinds of living optical imaging.
Directions for Use
Instructions for use of living skull clearing reagent
1. This product is divided into two parts, S1 is colorless clear liquid, S2 is slightly viscous colorless clear liquid.
2. Main components: S1 Main components are water, ethanol, urea and so on. The main components of S2 are water and sodium dodecyl benzene sulfonate.
3. Stored at room temperature, shelf life is 6 months, please use as soon as possible after opening the cover. If there is trace rod-shaped crystal precipitation at the bottom of S1, it is a normal phenomenon and does not affect the use.
4. The mouse scalp is depilated before use, and the scalp is cut open after depilation to expose the skull.
5. Remove the membrane on the skull surface with cotton ball and wait for the skull to dry, or dry the tissue fluid on the bone surface with ear syringe.
6. The cranial window plate is superglued to the skull to expose the target area.
7. Add 1-2mL S1 to the target area dropwise, keep S1 covered on the skull, and let stand for 10-15 min.
8. During the standing process, a small amount of cotton balls can be used to massage the skull with tweezers. (It is recommended to carry out under the asana mirror)
9. Replenish S1 in time to keep the skull covered.
10. A degree of transparency of the skull should be observed after S1 treatment.
11. Remove S1 with cotton ball, drop 1-2ml S2, keep S2 covered on skull, and let stand for 3-5min.
12. At the end of the experiment, the cranial window plate is removed, using tweezers to gently scrub the skull to remove the remaining super glue.
13. Wipe the skull with normal saline, suture the scalp, sprinkle some penicillin powder as appropriate.
14. For black mouse, because there are blood vessels in the skull, if the cortex needs to be observed, it is recommended to use cranial drill to partially thin the skull until the blood vessels in the skull can not be seen, and then clear it.
